Capillary Electrophoresis/Mass Spectrometry:
Course Description:
This one day course is an introduction to CE/MS and its capabilities
to solve real world problems related to biochemistry, pharmaceutical,
clinical and environmental analyses. It covers the different types
of CE/MS interfaces (including direct coupling using nano-tips);
nebulization, desolvation and gas phase chemistries associated
with electrospray ionization. The criteria for selecting solvents
and buffers for MS and CE separation suitability will also be
covered. The course will discuss the interpretation of spectra
produced by molecular peptides, proteins, oligonucleotides and
substances of pharmaceutical and environmental significance; sample
preparation and extraction / cleanup methods to improve concentration
detection limits. The course will also address CEC/MS and the
use various mass analyzers (e.g. quadrupole, triple quadrupole,
ion trap and time of flight MS) for qualitative and quantitative
CE/MS.
Course Outline:
Introduction Into CE/MS
Electrospray Mass Spectrometry:
Theory of Ionization
Ionization in Solution
Ion Evaporation
Gas Phase Chemistry
Sample and Solution Considerations
Sample pKa
Solvent
Buffer/pH/Additives
Flow Rate· Interpretation of Electrospray Spectra
High Molecular Weights
Peptides/Proteins
Oligonucleotides
Low Molecular Weights, Generating CID Spectra
Pharmaceuticals
Environmental
Quantitation
Coupling of CE with MS:
Interfaces
Layer Flow
Liquid Junction
Direct (u-tip)
On-line CE/MS
Electrolyte Considerations
Solvents Buffers/pH
Liquid Flow Rates
Sample Preparation
Extraction/Cleanup
Methods to Improve Concentration Detection Limits
What to Do When Incompatibilities Exist Between CE and MS
Electrolyte Phase Compatibilities
Liquid Flow Rate Incompatibilities
Layer Flow Addition
Starting Over: Developing a New Separation
Examples of CE/MS Analysis
Summary/Concluding Remarks:
Problem Set Reviewing Material Covered in Course
Appendix of CE/MS related references
Student Profile:
- This course is designed for the student who has completed
an LC/MS short course or who has working knowledge of LC/MS
and wants to improve their qualitative interpretation skills
for problem solving in LC/MS.
